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Poersch-Bortolon,Liane Balvedi; Scagliusi,Sandra Maria Mansur; Yamazaki-Lau,Elene; Bodanese-Zanettini,Maria Helena. |
Abstract The objective of this work was to analyze the androgenic response of Brazilian wheat genotypes to different pretreatments of the spikes, prior to the culture of isolated microspores, and to the effect of a gelling agent in the induction culture medium. Five genotypes were evaluated for embryo formation, green plant regeneration, and spontaneous chromosome duplication. Wheat spikes were subjected to two pretreatments: cold, at 4ºC for 21 days; and 2-hydroxynicotinic acid, at 32ºC for two days. Culture media were evaluated with or without Ficoll as a gelling agent. Cold produced more embryos and green plants than the chemical pretreatment in four out of five genotypes. Only two genotypes treated with 2-hydroxynicotinic acid were able to produce... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Triticum aestivum; Albinism; Androgenesis; Doubled haploid; Isolated microspore culture; Recalcitrance. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016001101839 |
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Poersch-Bortolon,Liane Balvedi; Pereira,Jorge Fernando; Nhani Junior,Antonio; Gonzáles,Hebert Hernán Soto; Torres,Gisele Abigail Montan; Consoli,Luciano; Arenhart,Rafael Augusto; Bodanese-Zanettini,Maria Helena; Margis-Pinheiro,Márcia. |
Abstract Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 454 sequencing; Candidate genes; RT-qPCR; Triticum aestivum; Water deficit. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400629 |
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